Effect of Adolescent Binge-Like Alcohol Exposure on Adult Alcohol Consumption in Sprague Dawley Rats

Usman Hamid, Cassie Chandler, Sarah Maggio, Hui Peng, James Pauly, Kimberly Nixon, Dr. Michael Bardo. Department of Psychology, University of Kentucky, Biomedical/Biological Sciences Research Building, 741 South Limestone Street, Lexington, KY 40536

Clinical studies indicate adolescent alcohol use is a risk factor for adult alcohol abuse. However, we recently found that rats exposed to ethanol (EtOH) via oral gavage during adolescence displayed reduced EtOH intake in adulthood compared to control-gavaged rats. Here, we tested whether this effect was due to a conditioned taste aversion (CTA) to EtOH or generalized neophobia. We hypothesized the reduced EtOH drinking was due to a CTA to EtOH resulting from the oral gavage. Adolescent Sprague Dawley rats received no binge, an EtOH binge, or a control binge every 8 hours for two days via oral gavage. Two-bottle choice sessions between water or a 15% EtOH, 0.2% saccharin/15% EtOH, or 0.2% saccharin solution began in adulthood. The non-water solution rotated every week and was presented in a counterbalanced order across groups. For all solutions, females drank significantly more than males. We found no evidence for generalized neophobia, but modest support for a CTA, as EtOH-binged rats consumed significantly less sweetened EtOH than control-binged rats, while no-binge rats fell in between. In males, a significant main effect of treatment group for 15% EtOH intake was driven by the control-binged rats drinking more than the no-binge rats. This unexpected result suggests the oral gavage may model chronic stress leading to increased adult EtOH intake, while including EtOH in the gavage may reverse this effect. The control-binged rats serve as a relevant behavioral phenotype for study, as adverse adolescent experience is also a risk factor for adult alcohol abuse. 

Additional Abstract Information

Presenter: Usman Hamid

Institution: University of Kentucky

Type: Poster

Subject: Psychology

Status: Approved

Time and Location

Session: Poster 11
Date/Time: Wed 3:00pm-4:00pm
Session Number: 7031