Characterization of a Portable, Customizable, Low-cost Temperature Control System for Amplification and Quantification of Specific Nucleic Acids in Microfluidic Devices

Mikayla Wood, Pablo Martínez Crus, Reha Abbasi, and Dr. Stephanie McCalla, Department of Chemical and Biological Engineering, Montana State University, 306 Cobleigh Hall, Bozeman, MT, 59717, United States of America

The ability to detect and quantify specific nucleic acids from patient samples is critical to the early diagnosis of many diseases, such as cancer. Current methods to quantity these specific nucleic acids require expensive, bulky equipment to control temperature during DNA amplification reactions. The aim of this research is to enhance and characterize the low-cost temperature control system developed in our lab, and to validate the system using a DNA amplification reaction to specifically detect a target nucleic acid sequence with a microscope. The temperature control system includes a holder that allows a microfluidic device to be continuously imaged and monitored under the microscope. The connection of the two thermocouples to the system were altered to improve the user interface and each thermocouple was calibrated individually to improve accuracy. The system controlled the temperature on a device made from a standard glass slide and PDMS, allowing a standard DNA amplification reaction (PCR) with 40 thermal cycles to be run. The difference between the initial and final fluorescence of the sample under the microscope were compared to the results obtained from the same sample in a commercially available temperature control system (Biorad CFX thermal cycler). A PCR reaction will be run and monitored in real time to obtain microscope images of a device throughout the amplification using the temperature control system. These results will confirm that the temperature control system can perform DNA amplification reactions as needed to detect and quantify specific nucleic acids. Future work includes using the temperature control system in conjunction with a 3D printed microfluidic device made without PDMS. Both of these technologies combined will allow for low-cost, compact, and robust temperature-dependent operations on microfluidic devices. 

Additional Abstract Information

Presenter: Mikayla Wood

Institution: Montana State University Bozeman

Type: Oral

Subject: Biological & Chemical Engineering

Status: Approved

Time and Location

Session: Oral 4
Date/Time: Tue 11:00am-12:00pm
Session Number: 403
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