Arbovirus Surveillance in Oklahoma

Christian Escritt, Caio Franca, Biology, Southern Nazarene University, 6729 NW 39th Exspressway, Bethany, OK 73008

The number of mosquito-borne diseases in the United States has drastically increased in recent years. Oklahoma has 64 known species of native and invasive mosquitoes from nine different genera that occur seasonally. Several of these species are medically important as they are vectors of diseases like West Nile Virus (WNV), Zika, and Chikungunya. Our goal was to determine vector mosquito abundance and diversity, and performed arbovirus surveillance in mosquitoes collected in Oklahoma. Mosquitoes were trapped using CDC miniature light trap, CDC gravid trap, and GAT trap across an urban-rural gradient in the Oklahoma City metro area. Collected mosquitoes were identified, females were sorted by species and pooled for arboviral RNA screening using RT-qPCR .A total of 75 trapping events took place from May to June of 2019, resulting in 3,178 mosquitoes trapped spanning 19 species from five genera​. Landscape played a major role in determining the abundance of the species collected and urban areas sustained a hotspot for mosquito diversity. ​Aedes vexans, Culex tarsalis, ​ and ​Culex pipiens/quinquefasciatus ​accounted for 37%, 20% and 15% of the total number of mosquitoes collected, respectively. The CO​2 baited CDC miniature light was the most efficient in trapping mosquitoes. Broad range primers were used to detect​ Flavivirus,​Alphavirus, ​and​ Bunyavirus in mosquitos trapped​. Samples tested positive for the ​Flaviviridae family were further analyzed using WNV specific primers. We detected 25 mosquito pools positive for ​Flavivirus, four of which were positive for WNV, in the early stage of WNV transmission season in Oklahoma. We detected the presence of arboviruses and determined the diversity of mosquito vectors in the greater Oklahoma City area. This active surveillance is relevant for public health applications and outbreak prevention efforts.

Additional Abstract Information

Presenter: Christian Escritt

Institution: Southern Nazarene University

Type: Poster

Subject: Biology

Status: Approved

Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3052