Investigating Key Enzymes Involved in Glypican-3 Release from Hepatocellular Carcinoma

Stevan Colakovic, Meaghan Keohane, Trishala Karmacharya, Dr. Samantha Katner, Department of Biochemistry, Chemistry, and Geology, Minnesota State University - Mankato, 228 Wiecking Center, Mankato MN 56001

Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. This form of cancer has a high rate of metastasis and recurrence, with only 10-20% of primary tumors in patients are usually resectable at the time of diagnosis. Uniquely, HCC contains significant amounts of glypican-3 (GPC3), a probable tumor biomarker in this type of cancer. GPC3 is a member of the glypican family, which are a family of heparan sulfate proteoglycans (HSPGs) anchored to the cell surface by a glycosylphosphatidylinositol (GPI) linkage. In general, glypicans act as coreceptors and interact with growth factors to regulate cellular growth activity. As GPC3 is significantly upregulated in HCC, GPC3 is known to heavily promote cancer progression and tumor angiogenesis (vascularization) in HCC patients. GPC3 shed from the cell membrane becomes soluble and can freely interact with other cells. Elevated levels of soluble GPC3 were frequently found in the extracellular microenvironment of HCC cells, and higher levels of soluble GPC3 are directly associated with poorer prognosis of HCC. Here, enzyme-linked immunosorbent assays (ELISA) and western blots were used to determine and compare the amount of GPC-3 shedding that occurred under different conditions. The purpose of this project is to identify key proteins involved in glypican-3 shedding from the HCC cell surface.

Additional Abstract Information

Presenter: Stevan Colakovic

Institution: Minnesota State University, Mankato

Type: Poster

Subject: Biochemistry

Status: Approved

Time and Location

Session: Poster 1
Date/Time: Mon 1:30pm-2:30pm
Session Number: 2164