Antioxidant Compound M-11 Inhibits the Growth of Staphylococcus aureus by Inhibiting DNA Replication

Grace Bybee, Faith Harvey, Casey Zoss, Bernice Semana, Nikolina Johnson, Dr. Paul Weber, and Dr. Daniel Jung, Department of Biology and Chemistry, Briar Cliff University, 3303 Rebecca St Sioux City IA 51103

Psoralea esculenta, found in the prairies of Iowa, contains the flavonoids genistein and daidzein. These compounds are known to exhibit high antioxidant activity. Previous research by colleagues at this institution have shown that an unreported component, M-11, isolated from this plant also exhibited potent antioxidant activity. Previous research in this lab revealed that the extract from Psoralea esculenta inhibits the growth of Staphylococcus aureus. Furthermore, Real time PCR analysis showed the decreased expression of the gyrase gene suggesting the decrease of bacterial DNA replication at 5 hours post treatment. The goal of current research is to characterize the kinetics of DNA replication inhibition by M11. The focus is how long it takes the M-11 compound to inhibit bacterial DNA replication. After treating S. aureus with M-11 for varying lengths of time, RNA is isolated from the treated cells. It is also isolated from a control group of untreated cells for comparison. cDNA is synthesized from the RNA of each group of cells. Real time PCR analysis uses specific primers: 16s ribosomal primer (as an internal control) and DNA gyrase primer. Gyrase expression will be determined by analysis of relative expression level (delta delta CQ). This kinetic data provides valuable information for the future use of extracts as a medicinal purpose.  

Additional Abstract Information

Presenters: Grace Bybee, Faith Harvey

Institution: Briar Cliff University

Type: Poster

Subject: Microbiology

Status: Approved

Time and Location

Session: Poster 8
Date/Time: Tue 5:00pm-6:00pm
Session Number: 5646