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Ronald Andanje, Dr. Amy Greene, Department of Biochemistry, Albright college 1621 N 13th street, Reading, PA 19604.
We studied the growth curve of Crithidia fasciculata. They have similar characteristics to Trypanosoma brucei which are the parasites responsible for the African Trypanosomiasis (sleeping sickness). Understanding how C. fasciculata grows and replicates may help us in understanding how its parasitic counterparts, Trypanosoma brucei rhodesiense and Trypanosoma brucei gambiense may behave and grow in humans. We used OD600 to measure its optical density in a spectrophotometer. We also used a hemocytometer to measure the cell count. We used data from both to come up with a formula to predict the cell number using the OD600 for future experiments. The formula was OD600 = 0.0513(cells *106). This formula also provided a faster way of finding out how they divided. We also found out that they doubled every 4.3 hours. We plan to use the data to find other ways as to how the cells would grow in response to oxidative stress from different components.
Presenter: Ronald Andanje
Institution: Albright College
Type: Poster
Subject: Biochemistry
Status: Approved
