PI3K Subunits in the Rat Supraoptic Nucleus

Riley Irmen, Zaynab Said, Duong Nguyen, Emily Leusink, Victoria Ihry, Austin Grove, Munir Isahak, Sara Whiteman, Marissa Andersen, and Jason Askvig, Department of Biology, Concordia College, 901 S 8th St. Moorhead MN, 56562

We previously demonstrated that the hypothalamic supraoptic nucleus (SON) undergoes a robust axonal sprouting response following unilateral transection of the hypothalamo-neurohypophysial tract in a young, 35-day-old rat. However, no collateral sprouting occurs following axotomy in a 125-day-old rat. Our lab has in vitro evidence demonstrating the role of CNTF (ciliary neurotrophic factor) in promoting process outgrowth via PI3K signaling. Thus, we compared the protein levels of the PI3K catalytic and regulatory subunits in the SON between 35- and 125-day rats. We found less protein of the catalytic domain, PI3K p110g, in the 125-day rat SON; however, we did not observe changes in the other three catalytic domains or the regulatory domains that we analyzed. Dual fluorescent studies demonstrated that all PI3K catalytic and regulatory subunits analyzed are localized to astrocytes and neurons in the SON. These data suggest that the lack of PI3K p110g found in the 125-day rat SON may prevent the collateral sprouting response from occurring, but future studies need to be performed to confirm this hypothesis.

Additional Abstract Information

Presenters: Riley Irmen, Zaynab Said, Duong Nguyen

Institution: Concordia College - Moorhead

Type: Poster

Subject: Biology

Status: Approved

Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3080