The Role of BK Channels Elucidated Through In-Vivo Pharmacological Manipulation

Lazaro Fernandez, Alejandro Suarez, Carolina Moncion, and Dr. Jorge Riera, Department of Biomedical Engineering, Florida International University, 11200 SW 8th St, Miami FL 33174

     In current literature, astrocytic neurovascular coupling is dominated by the arachidonic acid production of calcium-dependent phospholipase A2 (PLA2). However, the presence of calcium-sensitive BK channels on the endfeet of astrocytes has led to some debate on their potential contribution to neurovascular coupling. Past studies have utilized in-vitro or in-situ protocols that have failed to replicate a realistic in-vivo setting. To evaluate the contribution these channels have on astrocytic neurovascular coupling, we have combined the use of an in-vivo ChR2-EYFP transgenic mouse model with pharmacological inhibition of the PLA2 domain. We hypothesize that this will isolate the BK-based vasodilatory response for evaluation independent of PLA2. Moreover, we anticipate that this protocol will produce results indicating that vasodilation from astrocytic BK channels cannot be ignored. This is crucial in light of recent studies evaluating extracellular potassium coupling and would suggest a more pronounced role for the BK mechanism within the astrocytic vasodilatory response.

Additional Abstract Information

Presenter: Lazaro Fernandez

Institution: Florida International University

Type: Poster

Subject: Biochemistry

Status: Approved

Time and Location

Session: Poster 1
Date/Time: Mon 1:30pm-2:30pm
Session Number: 2172