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Wearable Biosensor for Monitoring of Inflammatory Biomarkers from Eccrine Sweat

Aashay Kothari, Badrinath Jagannath, Dr. Shalini Prasad, Department of Bioengineering, The University of Texas at Dallas, Tx 75080 Dr. Sriram Muthukumar, EnLiSense LLC, Allen, TX 75013

Abstract:

Human immune response involves the release of inflammatory cytokines due to an infection or an inflammatory event. These cytokine markers play an important role in the manifestation and progression of infection/inflammation. Therefore, real-time continuous monitoring of cytokines can aid in better prognosis, disease management and personalized treatment. However, current testing methods are based on blood sampling where real-time monitoring is not feasible and requires trained personnel. Alternatively, passive eccrine sweat has shown great promise for non-invasive and continuous monitoring that can be integrated into a wearable device. In this work, we demonstrate the detection of two key inflammatory cytokine markers, interleukin-6 (IL-6) and interleukin-10 (IL-10) from eccrine sweat towards real-time tracking of an infection. The developed wearable biosensor is sensitive and specific to both pro and anti-inflammatory cytokines. A metal semiconducting electrode was deposited on a porous polyamide substrate where, sweat would be absorbed and wick through. Specific capture probe antibodies in Choline dihydrogen phosphate ionic liquid solution was then individually functionalized on the electrode to capture the target analyte (IL-6 or IL-10) antigen in the sweat and measured using electrochemical impedance spectroscopy. A calibration curve was developed which demonstrated that the change in impedance response increases with increasing concentrations of the target analyte, confirming that we can reliably detect the concentrations of IL-6 and IL-10 in sweat. A limit of detection of 0.2 pg/mL and a wide dynamic range of 0.2- 200 pg/mL for both target biomarkers was achieved. Further, cross-reactive studies demonstrated that the sensor has no crosstalk to non-specific molecules. The developed sweat sensing device is envisioned as a wellness device to empower subjects for real-time monitoring of their health status and to allow physicians to provide patient-centric therapeutic intervention during inflammation or infection.




Additional Abstract Information

Presenter: Aashay Kothari

Institution: University of Texas at Dallas

Type: Poster

Subject: Biological & Chemical Engineering

Status: Approved


Time and Location

Session: Poster 2
Date/Time: Mon 3:00pm-4:00pm
Session Number: 2536