Investigating the Role of TAS3 in Root Nodulation in Trifolium Pratense

Caleb Gooden and Dr. Arthur Hunt, Department of Plant and Soil Sciences, University of Kentucky, 301A Plant Science Building, 1405 Veterans Drive, Lexington, Kentucky, 40546.

Root nodulation is a process recognized among legumes in which host roots are infected with a soil bacterium resulting in nodules on the root system. These nodules fix nitrogen from the atmosphere, and in agriculture, nitrogen-fixing crops help to reduce the need for fertilizer and are thus part of sustainable practice. Studies in Medicago truncatula, a nitrogen-fixing legume, have shown the process of root nodulation to be suppressed by the expression of a regulatory RNA (TAS3). The TAS3 gene promotes the production of lateral roots, secondary roots that extend from the primary system, by blocking transcription factors responsible for developing nodules. Thus, TAS3 is an attractive target for manipulation to improve nitrogen fixation in legumes and enhance their agronomic value. In Arabidopsis, TAS3a transcripts may end at one of two alternative polyadenylation sites, the choice of which determines whether functional or non-functional TAS3a RNAs are made. We hypothesize that if two polyadenylation (poly(A)) sites are present in the TAS3 region of Trifolium pratense (red clover), then differential use of these sites will be seen in lateral roots and nodules. Reverse transcription of T. pratense RNA was conducted to obtain concentrated cDNA to be used as a PCR template. 3’-RACE will be subsequently used to identify and sequence different TAS3 poly(A) sites. We expect the that the proximal site (that yields non-functional TAS3) will be utilized in nodules. If so, we will move forward by conducting agrobacterium mediated transformation of a gene knockout into T. pratense to further define the roles of TAS3 in nodulation in clover. 

Additional Abstract Information

Presenter: Caleb Gooden

Institution: University of Kentucky

Type: Poster

Subject: Biology

Status: Approved

Time and Location

Session: Poster 2
Date/Time: Mon 3:00pm-4:00pm
Session Number: 2623