Analysis of Gene Duplication in Arabidopsis Thaliana on Growth Patterns, and It's Application in the Agricultural Industry

Chuanzhu Fan, Feng Tao, William Nauam, Department of Biology, Wayne State University, 5047 Gullen Mall, Detroit MI 48202

Arabidopsis Thaliana has been a long time genetic model organism used to study genomics and genetics. Like many model organisms today, the genome has been entirely sequenced, and further analysis of this organism is now being pursued. The purpose of this study, through a series of phenotypic analyses, is to better understand the attributes of the genes of interest. Unknown functions in growth patterns, and requirements for proper mature growth has the potential to be useful in the agricultural industry. With the use of phenotypic analysis, we can best determine the role of gene function. There are two stages employed in the analysis, the first being logging of growth patterns of the leaflets into well-defined stages, in order to keep track of growth. The second stage being counting of flowers as they appear, is to account for and analyze growth patterns in mature stages of the plants life cycle in accordance to the genes studied. The mutant strains being tested for and studied are denoted as SSN1, and SSN2, with a control wild-type, Columbia (Col-0). SSN1 and SSN2 are duplicated genes and are paralogs. SSN1 is a duplication for the gene AT5G12960 and SSN2 a duplication in the gene ATG12950 . Through the observation and cross colonization of the Col-0, SSN1, and SSN2, we can observe how the gene duplication can affect the overall fitness of the organism and similar species. However, in the case where there is no statistical difference, further testing may prove that the gene in question may have some importance in mitigating shock in extreme cold or heat. We hypothesize that the gene duplication observed in this experiment will show the role it has on adaptation in this species and those similar, as well as its role in changing the mechanisms of existing genes.

Additional Abstract Information

Presenter: William Nauam

Institution: Wayne State University

Type: Poster

Subject: Plant Sciences

Status: Approved

Time and Location

Session: Poster 9
Date/Time: Wed 12:00pm-1:00pm
Session Number: 6172