Benzyl Butyl Phthalate-Induced Adipogenesis in 3T3-L1 Cells

Megan Daubenspeck, Dr. Thomas Peeler, Department of Biology, Susquehanna University, 514 University Avenue, Selinsgrove PA 17870

Obesity is a growing concern worldwide, as 83% of men and 72% of women are now considered overweight or obese just in the United States. The process of adipogenesis (fat cell development) can be studied in the 3T3-L1 cell model system, a mouse fibroblast cell line that can be induced to become fat cells by adding 3-isobutyl-1-methylxanthine (IBMX), insulin, and dexamethasone (MDI media). Phthalates are plasticizers used in a wide variety of products. They are also believed to be endocrine disrupting chemicals (EDCs) which interfere with the peroxisome proliferator-activated receptor γ (PPARγ) pathway involved in adipogenesis. We wanted to analyze the effects of two phthalates, Benzyl butyl phthalate (BBP) and Bisphenol A (BPA), on adipogenesis in 3T3-L1 cells. 3T3-L1 cells were stimulated with MDI induction media along with either BBP or BPA at concentrations of 10 μM, 50 μM, and 100 μM. Oil Red O staining was used to quantify the levels of adipogenesis following treatment with BBP and BPA. BBP was shown to increase adipogenesis at concentrations of 10 μM and 50 μM, while BPA did not promote significant differentiation of preadipocytes. Therefore, significant exposure to environmental phthalates, such as BBP may contribute to the increased rates of obesity. Additional experiments will be done to determine the optimal concentration for BBP-induced adipogenesis. We will also use Western blot analysis to determine the expression of PPARγ and C/EBPα, key transcription factors in the adipogenesis process, to learn more about the mechanism of phthalate effects on adipogenesis. 

Additional Abstract Information

Presenter: Megan Daubenspeck

Institution: Susquehanna University

Type: Poster

Subject: Biology

Status: Approved

Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3040