Genotyping Fatty Acid Variants in Arachis hypogaea

Hannah Curtis and Dr. Julie Marshall, Department of Biochemistry, Lubbock Christian University, 5601 19th Street, Lubbock TX 79407

In recent years, advances surrounding genetic modification have prompted further research into genome editing of plants and foods.  Nutritively, soybeans and peanuts are important due to the healthy fats they provide.  Previous studies have utilized soybeans to investigate levels of palmitic acid, which has been found to be linked to increased health benefits and shelf stability.  Soybeans that have higher palmitic acid contents have been identified as containing polymorphisms that increase palmitic acid levels, but it is currently unknown whether peanuts contain the same polymorphisms.  A gene involved in fatty acid synthesis which has an effect on the levels of palmitic acid in Arachis hypogaea, is β-ketoacyl-acyl carrier protein synthetase II (KAS-II).  If mutations cannot be found in the KAS-II gene, CRISPR-Cas9 technology could be utilized to generate mutations which could have a desirable impact on fatty acid levels.  This study has a two-fold mission of determining optimal growing conditions for A. hypogaea in order to develop a functioning genome-editing system as well as sequencing the KAS-II gene for specific polymorphisms that would alter enzymatic activity and thus, levels of palmitic acid.  DNA was isolated from A. hypogaea and primers were designed based on current KAS-II genetic sequences.  The DNA isolates and designed primers were run through polymerase chain reaction (PCR) to be amplified and this was followed by quantification of the PCR product.  Samples were then chosen to be run through a 1% agarose gel in order to excise bands and be purified for sequencing.  Sequences obtained were compared to the KAS-II gene sequence retrieved from the National Center for Biotechnology Information (NCBI).  The collected sequences will provide vital information on the presence of polymorphisms in the KAS-II gene which has further implications for positive effects on palmitic acid levels and subsequent increased health benefits in A. hypogaea.

Additional Abstract Information

Presenter: Hannah Curtis

Institution: Lubbock Christian University

Type: Poster

Subject: Biochemistry

Status: Approved

Time and Location

Session: Poster 1
Date/Time: Mon 1:30pm-2:30pm
Session Number: 2128