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The Effect of Coffee Extract on Adipogenesis

Jaden Carper and Dr. Thomas Peeler, Department of Biology, Susquehanna University, 514 University Ave, Selinsgrove PA 17870

The prevalence of obesity in the United States has increased from 30.5% in 1999 to 42.4% in 2018. Fat cells are created through a process called adipogenesis; in adipogenesis, a preadipocyte, which is an undifferentiated fibroblast, is differentiated into an adipocyte, which is a mature fat cell. Peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein a (C/EBPa) are two transcription factors that play crucial roles in the process of adipogenesis. Prior research has shown that coffee extract can inhibit the adipogenesis pathway and decrease the number of adipocytes formed in mice. 3T3-L1 preadipocytes are embryonic fibroblasts derived from mice and are used as a model system to study adipogenesis in vitro. 3T3-L1 preadipocytes were differentiated into adipocytes using MDI induction media (IBMX, dexamethasone, and insulin), insulin media, and 10% FBS/DMEM. In half of the wells, 5% Starbucks Pike Place Roast coffee extract was added during each differentiation step. Oil Red O staining was used to quantify the levels of adipogenesis in each of the treatments. Treatment with coffee extract inhibited the rate of adipogenesis compared to cells treated with the normal differentiation regimen. Previous studies have published conflicting results concerning whether caffeine is the compound responsible for the inhibition of adipogenesis. To clarify the results, we plan to add decaffeinated or caffeinated coffee extracts to 3T3-L1 cells during the adipogenesis process, and determine the effects on adipogenesis using Oil Red O staining. Western Blotting will then be used to determine whether expression of PPARγ and C/EBPa have decreased in caffeinated samples, which may suggest a mechanism for the effects of coffee on adipogenesis. By learning more about the process of adipogenesis, we may better understand the causes of obesity, and develop treatments that could slow the growth of this heath crisis.




Additional Abstract Information

Presenter: Jaden Carper

Institution: Susquehanna University

Type: Poster

Subject: Biology

Status: Approved


Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3026