Influence of Parkin W402A Mutation in Mitochondria Within Mice: A Morphological and Morphometric Approach

Luis Daniel Estrella, Benjamin Lamberty, Steven Totusek, Kelly Stauch, Howard Fox*. University Of Nebraska Medical Center. Department of Neurological Sciences. 601 S Saddle Creek Rd Omaha, NE 68106. Wayne State College. Wayne, NE 68787.

Parkinson's Disease (PD) affects 60,000 Americans annually. To improve treatments, it is essential we first have a better understanding of the disease and its characteristics. The PINK1/PARKIN pathway is believed to be an essential tool that cells utilize for mitochondrial repair. It is hypothesized that the disruption of this pathway is correlated with the formation of PD-like symptoms. Mutations in the genes involved can alter the pathway , which could cause disruption of mitochondrial physiology. Mutations of PINK1 & PARKIN have been documented but an understanding of their effects is lacking. Identification of the mutated proteins within cells is important to then be able to study each one and the characteristics and behaviors that will express in the protein itself. This study focuses on the mutation PARKIN W402A. The mutation is hypothesized to produce an overactive form of PARKIN within cells. This project used morphological images of mitochondrial networks to get qualitative measurements and performed morphometric measurements using MitographTM software to test for Mitochondrial response to specific stressors(CCCP). This behavior could not be confirmed due to the low N-value that was used in this project so more exhaustive research is needed and this accounts for only one of many mutations that should be examined for impact on mitochondrial morphology. Continuing research is being conducted to increase the sample size and test it with additional treatments. This research was made possible by grants from the National Center for Research Resources (5P20RR016469) and the National Institute for General Medical Science (NIGMS) (8P20GM103427), a component by the National Institutes of Health (NIH).

Additional Abstract Information

Presenter: Luis Daniel Estrella

Institution: University of Nebraska

Type: Poster

Subject: Biology

Status: Approved

Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3110