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Isolation of Circulating Lung Tumor Endothelial Cells Using Microfluidic

Jacob Hooper and Dr. Alhilal Taslim, Department of Pharmacy, 500 W University Ave, El Paso, TX 79968

     Lung cancer is one of the leading causes of death when it comes to cancer. This is due to the detection system being subpar. Meaning that the current detection systems are unable to see or fully diagnose the issue. The current method of using liquid biopsies are used to help find cancer at earlier stages via circulating tumor DNAs from blood tests, but epigenetic changes cannot be tracked or seen using liquid biopsies. Endothelial cells that are found within the tumor are useful and show pertinent results. Although collecting them from biopsy samples is not feasible. Which would create our question of can smaller amount of tumor DNA lead to a more functional diagnosis. 
     Tumor CECs come from damaged tumor vessels into blood which may hold a diagnostic biomarker and identification for lung cancer patients. They increase in lung cancer patients. With that being said these CECs are very hard to reach, which we plan to develop a CEC- capturing herringbone chip (HB-chip). We will coat the HB-chips with an antibody against a prion-like protein doppel that specifically expresses in pathological angiogenesis. Using the microfluidic device, we propose to isolate CECs from whole blood of NSCLC patients and assess whether these cells are valid biomarkers. 
     During this time, we have been in a process of developing the microchip that is comprised of a 1x3-inch glass slide which contain two channels on the upper surface engraved with 310 HB patterns that are edge to edge. We have also been working on the seeding process of the cells. After these processes the chip surface will be coated by a nanofilm by coating it with positively charged poly diallydimethlammonium chloride polymer, negatively charged biotin-labled alginate, streptavidin-Dylight 650 and finally biotin labeled antibodies. 




Additional Abstract Information

Presenter: Jacob Hooper

Institution: University of Texas at El Paso

Type: Poster

Subject: Biology

Status: Approved


Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3100