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Validation of Laboratory Developed Test for the Detection of Polyagglutination

Braxton C. Campbell, Victoria Jones, Ryan C. Kohli, Justin R. Rhees, Dr. Matthew J. Nicholaou, Medical Laboratory Sciences, Weber State University, 3875 Stadium Way, Ogden, Utah 84408-3905

The T-activated form of polyagglutination (PA) is a rare condition caused by certain microorganisms that can result in abnormal agglutination of red blood cells (RBCs). Although the condition is rare, there are currently no available commercial testing kits in the United States to use if a patient is suspected of having the disorder. Researchers at Weber State University developed a process to create a Laboratory Developed Test (LDT) kit by isolating a lectin that can detect the T-activated form of PA, which can easily be replicated by laboratories in a cost-effective manner. In this method, human RBCs are used to create the control cells by modifying the surface proteins to activate the T cryptantigen, which imitates what patient cells undergo when infected with the pathogens that cause T-activated PA.  The previous study utilized group O Rh negative blood cells to create their positive control cells. This study will build upon the previous work by validating the methods and expanding the options for creating the control cells. By testing donor blood segments stored in various additive solutions, as well as recovered, de-identified blood samples collected in ethylenediaminetetraacetic acid (EDTA), we will evaluate the quality of the control cells obtained from these diverse sources. This will provide further insight into the creation of the positive control cells in the LDT with various blood types. We surmise that, regardless of the blood type or additive solutions the RBCs are stored in, we will be able to create the positive control cells that produce strong agglutination when used with the isolated lectin. If successful, this will allow laboratories to create the LDT kit with various types of blood, acquired from either donor blood segments or from EDTA tubes.




Additional Abstract Information

Presenters: Braxton Campbell, Ryan Kohli, Victoria Jones

Institution: Weber State University

Type: Poster

Subject: Health & Human Development

Status: Approved


Time and Location

Session: Poster 7
Date/Time: Tue 3:30pm-4:30pm
Session Number: 5146