Construction of Candida albican specific antibody linked to antifungal peptides

Peter Atyia, Glory Nguyen, Frank Albano, Alexa Dickey, and Dr. Mason Zhang, Department of Microbiology, California State University Long Beach, 1250 Bellflower Blvd, Long Beach CA 90840

Candida albicans is the most common fungal human pathogen for life-threatening hematogenously disseminated candidiasis. Drugs for candidiasis are limited, and anti-drug resistance is on the rise. Therefore, it is important to develop alternative treatments for this disease. This study proposes an alternative treatment for candidiasis. The aim of this study is to use the specific human anti-Candida antibody M1 Fab to target chitinase or microbial peptide to C. albicans for killing. Chitinase is an enzyme that directly hydrolyzes the fungal chitin in order to kill the cell; the human form has been identified as chitotriosidase and will be used in this study. An antimicrobial peptide can disrupt the plasma membrane of fungal cells. This study will link the gene for the antibody M1 Fab to the gene of either chitotriosidase or an antimicrobial peptide, and the gene constructs will then be introduced into Escherichia coli for synthesis of the antifungal peptide-antibody hybrid proteins. The killing effects of these hybrid proteins will be studied against C. albicans. These antifungal peptide-antibody hybrids may be a potential alternative to conventional anti-Candida medications.

Additional Abstract Information

Presenters: Peter Atyia, Alexa Dickey, Glory Nguyen, Frank Albano

Institution: California State University - Long Beach

Type: Poster

Subject: Microbiology

Status: Approved

Time and Location

Session: Poster 8
Date/Time: Tue 5:00pm-6:00pm
Session Number: 5638