Antibacterial Effects of Organosulfur Compounds That Have Anti-cancer Potential

Kaitlyn Accardi and Dr. Niharika Nath, Department of Biological and Chemical Sciences, New York Institute of Technology, 1855 Broadway, New York, NY 10023

Cancer is a devastating disease. There are various compounds that are used for the treatment of cancer, and more candidates are being screened every year for potential anticancer activity. Sulfur containing compounds are gaining attention for their potential against cancer cells and some are additionally antimicrobial also, such as those in garlic. Organosulfur compounds (OSCs) contain sulfur and have an extraordinary range of chemical structures and activities, and many of these also have biological activity, such as anti-proliferative activity. Our overall arching goal is to examine anti-tumorigenic activity and antibacterial activity of OSCs that have the potential to release H2S. The purpose of my study was to focus on thirteen selected OSCs exclusively for antibacterial activity and to examine if they release H2S, and additionally, if there is a relation between H2S release and antimicrobial activity. We performed disc diffusion assays using various concentrations of OSCs against a variety of bacteria (Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa) with positive and negative controls. In addition to plating, we grew liquid cultures of Klebsiella pneumoniae and plotted growth curves from the optical densities when treated with the OSC. H2S release was measured using methylene blue in an in-vitro kinetic assay and absorption spectra was collected. We found that the OSC named 3- (methylthio) propyl isothiocyanate exhibited concentration-dependent antimicrobial effects against E. coli and K. pneumoniae. However, the kinetic assay of H2S release was found to not correlate with the antimicrobial effect by this assay. These OSCs will be continued for screening for antiproliferative effects in future studies, and there continues to be potential in these naturally occurring compounds. 

Additional Abstract Information

Presenter: Kaitlyn Accardi

Institution: New York Institute of Technology

Type: Poster

Subject: Biology

Status: Approved

Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3088