Assessing the Contribution of Alternative Splicing to the Regulation of SUMO3 SUMOylation in Human Cells

Andrea Garcia, Dr. Rosas-Acosta, Department of Biological Sciences, University of Texas at El Paso, 500 W University Ave, El Paso TX 79968

SUMOylation is a post-translational modification consisting of covalent conjugation of ubiquitin-like proteins called SUMO (Small Ubiquitin-like Modifier)-1, SUMO-2 and SUMO-3 to target proteins and regulate a wide array of cellular processes (Tempé et al., 2008). It has been proved that SUMO increases when cells experience any kind of stress, such as arsenic poisoning, hibernation, heat shock, any kind of ischemic events, and plays important roles for numerous viruses during infection. This study was designed to characterize the normal levels present in the cell of the two mature mRNA variants coding for SUMO3, produced by alternative splicing, and how their abundance is affected upon different types of stress, including hyperthermia, heat-shock, and infection with influenza A virus. To accomplish this, we used quantitative reverse transcription, polymerase chain reaction (PCR) using a template of RNA purified from stressed and normal cells. For the protein analysis, western blots were conducted to demonstrate the increase of SUMOylation on cells under the different stress conditions used. It is expected that SUMO 3 will have a positive effect and will increase SUMOylation under these conditions. Proving this will help the development of chemicals capable of regulating the cellular levels of the main SUMO modifiers in the cell which could in turn used as SUMO-targeted therapies for the treatment of conditions exhibiting huge morbidity and mortality, including strokes, heart attacks, and influenza infections.

Additional Abstract Information

Presenter: Andrea Garcia

Institution: University of Texas at El Paso

Type: Poster

Subject: Biology

Status: Approved

Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3015