A novel laser enameloplasty technique for caries prevention

Borisiuk, O; Tanweer, A; Graca, J; Levere, E; Arany, P.R. University at Buffalo Department of Oral Biology Foster Hall University at Buffalo, South Campus Buffalo, NY 14214

Objectives: Tooth decay (caries) is the most common human disease, and dental sealants are currently the most common treatment to prevent carious lesions. However, they are prone to limitations such as micro-leakage leading to secondary caries and wear or loss of retention and require replacements. The objective of this project is to examine if a novel laser enameloplasty technique would be a more efficacious and cost-effective approach for caries prevention. 

Methods: Following UB IRB (#STUDY00001193) approval, extracted human teeth were secured in polymethylmethacrylate (PMMA) blocks to enable performing procedures and sectioning for scanning electron microscope (SEM) analyses post-treatments. Occlusal surfaces were treated by an Er,Cr;YSGG (Waterlase, Biolase) laser and its effects on the depth and uniformity on resulting fissures were examined. As food and bacteria are entrapped in the deep, narrow occlusal fissures, we developed a novel slime-retention toothbrush simulator assay to examine the efficacy of laser-widened fissures. We used fluorescence imaging and digital quantitation for analyses.  

Results: We observed that laser enameloplasty, at specific settings, effectively ablated and conservatively widened occlusal fissures that reduced slime retention compared to untreated surfaces. 

Conclusion: This laser enameloplasty technique could prevent occlusal caries and have a significant impact on clinical dentistry. Ongoing work in the lab is also examining combination of the laser technique with fluoride applications to further improve term caries prevention. These prophylactic approaches can have a major impact in preventive dentistry as it would improve efficacy and access to care.      

Acknowledgements: We would like to thank Mr. Peter Bush, SCIC and Steven Makowka, Dental Materials Lab for technical assistance. Support for this project was provided by UB SDM start-up funds to Arany lab.

Additional Abstract Information

Presenter: Oleg Borisiuk

Institution: University at Buffalo, State University of New York

Type: Poster

Subject: Biology

Status: Approved

Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3114