Biological Analysis of Glutathione Attached Fiber for Potential Skin Graft Generation

Karim Kari, Niyaf Alkadhem, and Dr. Morshed Khandaker, Department of Engineering & Physics, University of Central Oklahoma, 100 N University Dr, Edmond, OK 73034

Abstract: Over the past three decades, significant progress has been made in the area of skin grafts using different types of tissue engineering scaffolds. However, there is no skin graft available in the market that is suitable for a patient with diabetes. The long-term goal of this study is to develop a skin graft that is suitable for patients with diabetes. The main goal of this study is to determine the effect of nanofiber mesh with an enzymatic antioxidant for the maintenance of skin repair process and reduce inflammation after in vivo implantation of the skin graft in a diabetic patient. The study is based on two research questions: (1) can daily intra venial (IV) injections of glutathione (GSH) reduce high oxidative stress in organs of the body? and (2) can GSH be attached to PCL nanofiber membrane (NFM) based skin grafts and act as an anti-oxidative drug releasing agent? The answers to the above questions will lead to the development of an anti-oxidant immobilized functional skin graft that has potential for skin repair of a diabetic patient. Our study will include testing GSH’s bacterial properties, measuring the amount of GSH released from the scaffolds, and to test blood sugar levels on mice that are fasting or diabetic in order to study the effects of glutathione. Our study will also include cell viability tests on the GSH-PCL scaffold which includes measuring the cell adhesion, proliferation, differentiation and focal adhesion tests on each of the scaffolds. The results of these tests will show us clinical potential of GSH-PCL scaffold as a skin graft. The above results will also conclude that GSH immobilized in PCL can be a suitable candidate for skin grafts in diabetic patients.


Additional Abstract Information

Presenter: Karim Kari

Institution: University of Central Oklahoma

Type: Poster

Subject: Biology

Status: Approved

Time and Location

Session: Poster 3
Date/Time: Mon 4:30pm-5:30pm
Session Number: 3094