Plant-associated Bacteria Compete Differently on Diverse Media Sources

Author: Thomas C Hyde Mentor: Sarah Lebeis, PhD Department: Microbiology Institution: University of Tennessee at Knoxville Institutional Address: Knoxville, TN 37996

The microorganisms that live inside and around plants must endure chemical and biotic stresses. Microbes compete at the root for resources provided by plants. In this study, we investigated microbe-microbe interaction phenotypes between a collection of environmental isolates that will be used for microbiome studies. Additionally, we performed in silico analyses of secondary metabolite potential. The microbe-microbe interaction assay was conducted on three types of media in order to determine if nutrient composition was the reason for inhibition between isolates. We conducted the experiment by plating a lawn of isolate on one of the three aforementioned media types. We then placed a 5ml bacterial spot on top of the lawn. The assays were observed for inhibition indicated by the formation of a cleared halo around the 5 ml bacterial spot. We screened 15 different isolates across different media sources and experimental setups, totaling more than 1,400 individual interactions. In conclusion, we saw that 6/15 isolates were able to inhibit other microbes, while 10 were inhibited by at least one other microbe. We correlated in vitro results to in silico predictions. We can use the competitive interactions to help us understand how microbes assemble into specific niches along the root. 

Additional Abstract Information

Presenter: Thomas Hyde

Institution: University of Tennessee at Knoxville

Type: Poster

Subject: Microbiology

Status: Approved

Time and Location

Session: Poster 8
Date/Time: Tue 5:00pm-6:00pm
Session Number: 5657