Creation of Unnatural Dipeptide Antibiotics Against Pseudomonas aeruginosa

Madison Blanton, Dr. Kristen Mudrack, Department of Chemistry, Milligan University, 1 Blowers Blvd, Milligan College, TN 37682

Cystic fibrosis is an autosomal recessive genetic disorder that affects the cystic fibrosis transmembrane conductance regulator protein. This abnormal protein prevents the proper movement of chloride ions across a cell membrane that will result in a buildup of mucus on the outside of the cell. This excess mucus will cover and suppress cilia on lung cells, preventing them from expelling foreign pathogens that enter the respiratory system. Without the body having the proper primary defense against bacteria, the lungs serve as the perfect breeding ground for microbes, specifically Pseudomonas aeruginosa. Once contracted, P. aeruginosa is likely to live within the patient for the longevity of his/her life. Today, there are two antibiotics available to combat P. aeruginosa. Patients are placed on a treatment regimen that requires them to alternate antibiotics month to month, allowing the bacteria to form a resistance to the medication. Ongoing research at Milligan University is working to combat antibiotic resistance by creating novel antibiotics to kill P. aeruginosa

Through dipeptide synthesis, various alkylating and acylating agents were combined to find the best combination to kill the bacteria. The compounds were then purified through filtration methods and tested using TLC. Novel antibiotics were tested against P. aeruginosa cultures through a bioassay to determine efficacy in comparison to currently used antibiotics on the market. Thus far, research shows that 4-flurobenzyl derivatives in combination with D-amino acids were the most effective combination. Currently, research is being conducted to determine if acylating agents with different halogens are more effective. 

Additional Abstract Information

Presenter: Madison Blanton

Institution: Milligan College

Type: Poster

Subject: Chemistry

Status: Approved

Time and Location

Session: Poster 4
Date/Time: Tue 11:00am-12:00pm
Session Number: 3606