How Does Atrazine Affect Corticosterone Stress Response in the Northern Two-lined Salamander (Eurycea bislineata)?

Darlene Slavick and Dr. Tanya Matlaga, Department of Biology, Susquehanna University, 514 University Ave, Selinsgrove PA 17870

Atrazine is a widely used herbicide that enters streams and serves as an abiotic stressor. Such herbicides cause chronic stress in exposed aquatic organisms and can contribute to local and global declines in amphibian populations. While studies have shown a relationship between atrazine exposure and dysregulated stress response in several amphibian species, there has been no such study on the northern two-lined salamander (Eurycea bislineata), commonly found in agricultural regions. Our study aims to determine the effects of atrazine on stress response in northern two-lined salamander larvae via water-borne collection of the stress hormone corticosterone. We predict that atrazine will alter corticosterone release in larvae exposed to higher concentrations of the herbicide, indicating a dysregulated stress response. Larvae were exposed to 0, 50, 100, 200 μg/L atrazine with 5 replicates per treatment for 14 days. We will validate water-borne corticosterone collection in this species via a standard agitation test. We will be analyzing the water samples for corticosterone levels using an enzyme-immunoassay kit in spring 2021. Examining the impacts of atrazine on stress levels of northern two-lined salamander larvae will provide insight into how to mitigate amphibian population decline.

Additional Abstract Information

Presenter: Darlene Slavick

Institution: Susquehanna University

Type: Poster

Subject: Ecology

Status: Approved

Time and Location

Session: Poster 5
Date/Time: Tue 12:30pm-1:30pm
Session Number: 4096